Prevent getting oxidation of Methionine during hydrolysis with 6NHCl under vacuum?

03 February 2016 1 2K Report

Prep sample for AAA analysis and recently big problem, Met keeps getting oxidized ...?

Hi there,

If your purpose is just quantitative AAA, total oxidization of Met into Met-sulfone by performic acid prior hydrolysis allows quantitative recovery of Met-sulfone after hydrolysis. Then quantification is made towards Met-sulfone standard.

For info:

http://www.ncbi.nlm.nih.gov/pubmed/19937719

http://www.acta.sapientia.ro/acta-alim/C1-1/alim1-5.pdf

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