Prevent getting oxidation of Methionine during hydrolysis with 6NHCl under vacuum?

03 February 2016 1 2K Report

Prep sample for AAA analysis and recently big problem, Met keeps getting oxidized ...?

Hi there,

If your purpose is just quantitative AAA, total oxidization of Met into Met-sulfone by performic acid prior hydrolysis allows quantitative recovery of Met-sulfone after hydrolysis. Then quantification is made towards Met-sulfone standard.

For info:

http://www.ncbi.nlm.nih.gov/pubmed/19937719

http://www.acta.sapientia.ro/acta-alim/C1-1/alim1-5.pdf

Badges
Science topic

More Zulaika Davison's questions See All

Pigeon behavioral researchers - Touchscreen autoshaping?

We're starting to use touchscreens with pigeons in an arrangement I have not used before, so I need your expertise. The arrangement is a large touchscreen at the other end of the experimental...

26 February 2020 6,860 5 View

Is there research demonstrating the invalidity of the concept of the personal clown?

I am interested in research which corroborates findings that show the invalidity of notions of 'personal clown' or 'inner clown'. This research mght, for example: - Suggest evidence for how the...

05 March 2018 1,237 2 View

Can anybody recommend a suitable supplier etc for water (bottled) to use in an incubator humidity tray?

Can anybody recommend a suitable supplier etc for water (bottled) to use in an incubator humidity tray (our purified source is not suitable because it is DI, so I'm hoping it will be easier to buy...

31 May 2015 3,001 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View