I'm trialing a method of culturing the plant microbiome and I'd like to know if a majority of the microbial cells will still be viable for culturing if I store the whole plant sample in a -80 degree freezer. Will I only be selecting for and ultimately culturing micro-organisms that can withstand -80 degrees, or will the microbiome still be as viable for culturing had I processed the samples before freezing.
It is more likely the former: you will perhaps be only selecting for those that can withstand -80 degC (likely those who can form spores[?], among others). A literature review is perhaps in order to give you an idea of what storage strategy to implement that will properly maintain microbiome diversity/strain representation in the sample.
by trails, and after in depth review of the literature we can finalize which which microbe will stand which temp.
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