The isolation of single cell suspensions from lymphoid tissues is straightforward incuding thymic tissue ( size decreases with age). A good example for a protocol is the following using cell strainers: https://www.thermofisher.com/de/de/home/references/protocols/cell-and-tissue-analysis/protocols/cell-preparation-flow-cytometery.html

Kind regards

Alois

Here is the link to publication for protocol of making single cell suspension from thymus .

https://doi.org/10.1002/0471142735.im0326s107

Thank you. This is very helpful.