Preparation of embryonic nuclear protein sample to do WB : blue chewing-gum after heating 96°C during 5min ???

27 September 2023 1 6K Report

I am currently trying to make concentrated samples with a hundred frog embryos to make western blots.

Everything is fine during protein extraction. However, when I add the laemmi buffer, I begin to have aggregates that form. Once heated, my samples turn into blue chewing gum impossible to resurface.

Has this ever happened to anyone?

Xi Jiang

Never worked with that kind of samples but when working with bacteria, the sample after heating with laemli buffer gets slimy because of the DNA in the sample. This problem is avoided when DNAseI is used during the protein extraction step (we use 5ug/ml DNAseI final concentration).

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