Dear all,
A protein enzyme has 20 amino acid as signal peptide sequence, which is located in the C-terminal. Is there any bioinformatic tool using for prediction of a type of secretion system that protein belongs to?
Hi Van, expasy has some useful tools.
https://www.expasy.org/resources/search/keywords:signal%20peptide
Thanks Steingrimur Stefansson
Hi there, I have been working with protein named X ( pI= 6.2 and MW =66 KDa). I obtained some protein crystals in the condition containing high salt NaCl (0.1M Bis-tris pH 5.5, 3M of NaCl), one...
10 November 2017 5,378 0 View
Hello everyone, I have been purifying protein A (pI =8.4) for crystallization. My problem is that this protein is precipitated during and after the concentracing process. Usually, the Tris-HCl...
07 August 2016 7,772 5 View
I have some issues with plasmid drawing for publishing. It is very grateful if anyone can suggest a suitable software for creating the plasmid map? Thank you in advance.
02 March 2015 6,946 5 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...
08 August 2024 4,842 0 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,218 4 View
Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...
05 August 2024 3,246 1 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...
04 August 2024 8,432 3 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?
31 July 2024 6,544 11 View