Is secondary fixation necessary for processing plant materials for SEM?

I am trying to create a protocol for several fixation methods for Scanning electron microscopy. using FAA, glutaraddehyde and ethanol as fixatives.

11 November 2019 7,215 1 View

What non-parametric statistical analysis can you use for small sample sizes, n=2, and still show statistical significance?

I am doing a project on a therapeutic group, utilizing non-parametric ordinal data to account for changes in emotional distress. Unfortunately attendance has been somewhat inconsistent throughout...

23 March 2019 9,948 14 View

How does the UVF parameters affect the quality of heavy oils?

I have data regarding UVF parameters, which include Fluorescence Intensity Values (i) in Relative Fluorescence Units (rfu). Attached in the excel file is the UVF parameter list and its standard...

16 May 2018 2,636 1 View

What is Mesenteric Cancer?

What is Mesenteric Cancer? What is the root cause of Mesenteric Cancer? How can we cure or prevent Mesenteric Cancer?

20 October 2017 5,744 4 View

How would I analyze and present longitudinal patient data, which is split into 2 or more samples?

12 July 2017 8,424 2 View

Effect of flash freezing of pelleted cells?

20 February 2017 7,949 2 View

How to directly test viral primary antibody?

I have a polyclonal anti-MODV antibody as well as a monoclonal anti-flavivirus (E protein) antibody and would like to test both against MODV in cell culture. In developing a protocol I would like...

14 January 2017 5,944 4 View

Can we use Big Data Analytics to Automate / Replace Manual Literature Review that are conducting by Researchers?

14 October 2016 6,049 7 View

Can we use Big Data Analytics to Analyze & Derive New Areas for PhD Research?

14 October 2016 6,636 13 View

Have anyone encountered PMMA cracking or charging issue on hBN flakes after development?

I create patterns (several micrometers) on hBN flakes on a Si/SiO2 substrate using EBL (100kV). I find cracking / extra lines emerging from my pattern after development. I guess it is due to...

02 July 2016 2,355 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

No title

01 March 2021 3,905 5 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

What is the optimum time and temperature of blanching and pre-frying potato for frozen fries?

I am helping a local home industry for developing frozen fries from their local farm. I want to know what is the optimum blanching and pre-frying temperature and time for processing. Please also...

23 February 2021 1,616 5 View