Pot experiment with different densities ( kg/ha) of plant species, Do i use number of seeds as given or i have to half number of seeds for one pot?

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Simultaneous imaging of multiple components (Carbohydrates, Fat, Protein) in food products using confocal microscopy??

Hi Everyone, I am trying to characterize the microstructure (localization of carbohydrates, fats, and proteins) of coconut milk powder using confocal microscopy. From the review of the available...

12 January 2021 5,464 2 View

Is it possible to separate Supleco 37 Fame mix using HP-5MS column?

Hi everyone, I am new to FAME analysis. I am trying to separate Supleco 37 Fame mix using the HP-5MS (30 m * 0.25 mm ID * 0.2 um thickness) column using the following protocol: System: Agilent...

19 December 2020 7,356 4 View

Transcription mediated amplification (TMA)?

I am trying to optimize TMA in my lab. When I start with 150 pg of DNA (Size 235 bp), I get good amplification, which I can visualize as an equivalent size dsDNA product, gel pic attached....

18 November 2020 7,622 3 View

How to do data analysis of a split plot design experiment with an extra control treatment outside the design but in the same field?

In an split plot experiment, 4 methods of Nitrogen(N) application was assigned in main plot and 3 doses of N in subplots and replicated thrice. Along with it, in the same field, No N control...

20 September 2020 9,944 2 View

How teachers are engaging their students at the time of COVID-19?

Most of the countries are still not opened the school, college and universities because of COVID-19. It is spreading rapidly. In such situation, what are the roles playing by teachers/lecturers to...

14 July 2020 5,998 30 View

How do we do intersection of two raster image in ENVI?

After threshold the value of Moran Geary and Getis index using Local statistics in Envi how we get the common part between Moran and Geary indices

05 April 2020 6,234 3 View

CAN anyone explain me about cell staining, egg staining by using different dye such as methylene blue?

what is the principle behind staining what is its applications? why eggshell does not stain? why RBS does not stain? please explain to me everything. thank you

23 February 2020 7,247 2 View

Could anyone free for english editing service ??

One of my research article is rejected due to english language from Parasites and Vectors journal?? Is there anyone who able to assist me??

29 December 2019 5,207 8 View

I'm having a hard time to get the UV-vis spectra of acetone. What may be the reason?

I'm getting a weird UV spectra for pure acetone. The analysis were performed on Shimadzu UV-vis spectrophotometer however the graphs weren't good with noise patterns as seen on the image. Any help...

11 November 2019 9,477 5 View

Is there a way to prevent agarose drop from floating after addition of media?

Hello everyone, My experiments require me to form a 0.5 wt% agarose with a certain chemokine on a 24 well plate. This would allow slower release of the chemokine and we want to check the...

19 August 2018 2,394 1 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

Recalcitrant seed short-mid storage method?

Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...

25 February 2021 2,442 1 View

Is there a suitable way to confine cell attachment area inside a multi-well plate?

Are there any types of cylindrical cell culture inserts that are designed to confine cell seeding area? Say, if I'm using a 12 well plate (3.5 cm^2/well), but only want to seed cells in the middle...

22 February 2021 7,127 4 View

Is it possible to use maize mature seed to do transformation for maize transgenic study?

Almost all research reports which i ever seen work on Early mature embryo for Agrobacterium mediated maize transformation. In other grass families like rice, we can use mature seed and what do...

17 February 2021 1,434 13 View

Dealing with BMDM seeding and RNA extraction ?

biomedical master student here , i am dealing with mice BMDM and would like some help , i seed 0.5 x 10^6 cells/per well in a 6 well plate but when i extract the RNA i get a very low yeild , i...

15 February 2021 1,102 5 View

Is secondary metabolite production more at vegetative growth stage or during reproductive stage?

Hi, I am working on the effect of heavy metals on a medicinal plant by pot experimentation. Analyzing the effect of heavy metals on secondary metabolites is one of the parameters. I will be...

11 February 2021 6,683 6 View

Are the topics below viable research in plant biotechnology?

1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.

04 February 2021 9,069 3 View

Seeds harvest from white clover by selfing?

I tried to get seeds from white clover by self-fertilization but I could not get the seeds. As you know the white clover is generally fertilized by crossing. I wanna get the seeds by...

04 February 2021 9,152 4 View

What is the best method of preparing cell supernatant samples for a Luminex assay to detect cytokines ?

I am planning to run a Luminex assay using cell supernatant to assess cytokine secretion by tumor cells. I came across different methods in literature; some suggest seeding in complete media and...

03 February 2021 4,867 3 View

How do I measure Arabidopsis germination rate?

Hi there, I'm looking for a way to measure the Arabidopsis germination rate, like what media I should use to plant the seeds and what indicator I should see for germination. I'm planning to...

02 February 2021 4,785 3 View