I am currently performing biofilm studies using the Calgary device (96-well plate with peg lid) and a non-motile bacterial species (S. aureus) but am having difficulty forming biofilms on the pegs.
I'm considering adding a few parameters to try promote adherence and biofilm growth, but first wanted to know if it is possible considering the bacterial motility?
Or should I opt for growing the biofilms within the wells rather than on the pegs?
Thanks!
It shall be worth to attempt the formation of biofilms on both peg and well-surfaces to find out the actual preference of the organism to create such niche. Streptococcus mutans does form biofilm/ plaque formation on teeth and is nonmotile. Adherence may be modulated/ promoted by pre-exposure of the surface with sterile solution of some sugars [dextrose, sucrose etc.], amino acids [Lysine] and ECM [Collagen, fibronectin, albumin etc.].
Bacterial mobility is only one aspect that contributes to biofilm formation
Although motility is a plus, yet biofilm formation is related to many adherens. So the answer is yes, non-motile bacteria can definitely form biofilms.
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