Kevin Masterson

5 Questions 4 Answers 0 Followers

Questions related from Kevin Masterson

Possible to grow biofilms with non-motile bacteria?

I am currently performing biofilm studies using the Calgary device (96-well plate with peg lid) and a non-motile bacterial species (S. aureus) but am having difficulty forming biofilms on the...

11 November 2019 9,486 4 View

Effects of TC treated and non-TC treated 96-well plates with bacterial strains?

I'm performing assays on a number of bacterial strains, some of which are capable of forming biofilms. What effects would a "tissue culture treated" plate and a "non-tissue culture treated" plate...

01 January 2019 8,678 6 View

What can cause lower absorbance readings from center of 96-well plate following MTT?

I have been running drug treatment assays on cell lines in 96-well plates. Following 72 hours incubation, I perform a MTT assay to assess cell viability following the treatment. I am frequently...

05 May 2018 3,324 5 View

How to prevent Silver Nitrate (AgNO3) precipitating in growth broth?

I am trying to determine the MIC of Silver Nitrate (AgNO3) against a number of bacterial strains using broth micro/macro dilutions method. I prepared the AgNO3 in distilled water to a...

01 January 2018 8,907 8 View

Salt-free bacterial growth media?

Hi all! I need a growth media with no salt, or as little salt as possible that can support a bacterial culture for 18 - 24 hours! Any suggestions of a broth I could purchase or even ingredients...

01 January 2018 8,266 3 View