I'm performing assays on a number of bacterial strains, some of which are capable of forming biofilms.
What effects would a "tissue culture treated" plate and a "non-tissue culture treated" plate have on bacteria suspensions??
Would a TC treated plate cause lower amounts of planktonic cells and/or promote biofilm formation as compared to non-TC treated plates?
Thanks!
Hello Kevin. My team compared the biofilms on different brands of 96-wellplates (TC-treated, non-TC-treated), and found that some non-TC-treated plates (super cheap) had the lowest biofilm of the same bacteria. We then concluded that non-biofilm experiments can be used on certain non-TC-treated plates, while biofilm experiments should be used on TC-treated plates.
Hello, I think that there will not be a big difference between the two materials (but this can be quickly tested). There are many factors that influence the degree of biofilm accumulation. The degree of biofilm-formation will depend on a range of factors, such as species (and strain), growth medium (pH and salts which will affect the relative hydrophobicity of the cells, the presence of proteins that might coat the surface of wells, etc) and incubation conditions.
Hope this info will help you.
Good luck!
Hello Kevin. My team compared the biofilms on different brands of 96-wellplates (TC-treated, non-TC-treated), and found that some non-TC-treated plates (super cheap) had the lowest biofilm of the same bacteria. We then concluded that non-biofilm experiments can be used on certain non-TC-treated plates, while biofilm experiments should be used on TC-treated plates.
Yes, good to know. Confirm your results in repeatability conditions it means several times to be sure in results and it will become good topic for publication.
Be careful to select those plates whose surface most closely mimic the conditions you want to test, or at least be sure to report what plates you use. If you want to test attachment to plastic or glass, use untreated plastic or glass. Surface chemistry matters so make sure you control this variable and report it in your methods.
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