I am working with a protein and trying to resolve it on Blue-Native PAGE. Can somebody suggest be a recipe for it?

I have been trying to perform Blue-Native PAGE using procedure from doi:10.1038/nprot.2006.62. However, the stacking gel does not solidifies even after two hours, at room temperature. Can...

04 April 2019 4,777 2 View

I am planning for Native PAGE for a protein with pI 8.5 - 8.7. What should be the minimum difference of pH of buffer to avoid electrode switching?

The Gel has pH 8.9. and the pH of loading dye is 6.67. What should be the pH of running buffer to allow smooth running of my protein into the gel. I prepared buffer of pH 8.49 but I was unable to...

25 March 2019 9,877 6 View

Any suggestion what's going on?

I submitted a manuscript on a novel and interesting finding on July 30, 2018 to a reputed journal. Following editorial review it went to peer-review. It is over 8 months, no decision yet. What...

24 March 2019 9,737 2 View

How to find the natural frequency of fixed thick glass?

Dear researchers, i am looking for equation to find the natural frequency for fixed thick glass. please suggest

28 November 2018 2,736 2 View

What are the advantages growth of GaN on graphene in optoelectronics?

I would like to write my research plan for my PhD on this topic.

21 April 2015 4,301 3 View

Is it compulsory/mandatory to examine the mediating effect in the conceptual framework that was analyzed utilizing AMOS?

Increased value of invertebrates - Mediating variable

08 April 2015 2,834 5 View

How is oil content estimated in mustard seed? Can anyone provide me a protocol regarding this?

I want to estimate the percent of oil present in mustard seed.

24 March 2015 2,233 7 View

What is the minimum sample size needed to conduct the SEM analysis utilizing AMOS software?

05 February 2015 9,648 7 View

How to stop dye transfer from one cell to another?

I am trying to optimize ADCC for HIV infected cells. I am labeling my targets with CFSE and any other membrane or nuclear dye. My effectors are unlabeled. The problem is the second dye, no matter...

15 January 2014 7,731 3 View

What is the scope of ethnobotanicals in the poultry nutrition?

Ethnobotanicals means herbs that are used by the natives for long time to treat differnet health issues. However some time there is no scientific justification available.

16 April 2013 7,103 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Is there a classification scheme for rock cavities or caverns with respect to their size and shape?

The range of forms and sizes of cavities in rock is very big. Is there a classification scheme for rock cavities regarding their size and shape?

02 March 2021 4,511 3 View