Positive control for exosome CD63 antigen: Do you know any mice cell line to be strongly CD63 positive?

22 November 2017 1 836 Report

I am working on western bloting to characterize mouse exosomes by CD63 anrigen. I performed It several times but couldn't get positive result. Because of limitations in the amount of the exosome preparation, for the test setting up, I am looking for a cell line which express CD63 strongly.

Is there any mouse cell line to be useful for this purpose?

André Cronemberger Andrade

To detect CD63 you have perform a western blot without redution conditions. In the sample buffer do not use DTT or 2-mercaptoethanol. For posite control you can use the cell lysate. CD9 and CD81 are markers for exosomes as well.

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