Anwar Fathollahi

6 Questions 4 Answers 0 Followers

Questions related from Anwar Fathollahi

How I can evaluate demyelination in the sections of mice spinal cord?

Hi I have very little experience with histology. I have sections of spinal cord of my test groups (EAE mice) which are stained by the luxol fast blue-cresyl violet. what is the characteristic or...

12 September 2018 6,641 4 View

What are these attached spindle-shaped cells to the plate wells surface?

Five days ago, I harvested splenocytes from C57BL/6 mice by 70 um cell strainer and in order to remove any adherent cells including macrophages, I cultured them in a treated 25 flask for 24 hours...

15 March 2018 9,204 1 View

How I can find out whether my CFSE labeled cells are proliferated and how much they are proliferated?

Hi everybody my question is about the analysis of the results. how I should analyse the data on the software and how the resultant output is interpreted? (how I should report it to others?) I have...

20 February 2018 7,967 5 View

Positive control for exosome CD63 antigen: Do you know any mice cell line to be strongly CD63 positive?

Hi I am working on western bloting to characterize mouse exosomes by CD63 anrigen. I performed It several times but couldn't get positive result. Because of limitations in the amount of the...

22 November 2017 875 1 View

Can my Blotting ECL signal vanish in a night?

Hi, I blotted my samples on nitrocellolose membrane last night and after adding ECL in the dark room, clearly saw light emission from the blots but had some problems and could'nt record my results...

15 November 2017 9,843 6 View

Can someone please share protocol for Apoptosis induction in MSCs (mesenchymal stem cells) by UV irradiation??

Can someone please share protocol for Apoptosis induction in MSCs (mesenchymal stem cells) by UV irradiation?? induction in other cell types can also be helpful. I am going to perform a short...

30 June 2015 5,925 2 View