Process analytical technology is applied in On-line HPLC which is used to measure the purity of biopharmaceuticals. The main keyword used in this article is POOLING. i.e. Online HPLC offers a feasible approach for analysis that can facilitate real-time decisions for column pooling. Feasibility of using ONline HPLC system for REAL TIME POOLING of process chromatography column. In the case of high resolution separation where a part of peak is being collected to pool the product and pool out the impurities.. Does anyone know what does the word pool refers to?
To put together fractions of the elution profile of a process chromatography. Ok? That?
Dear Vjosa,
To understand the pooling terminology I would like to give you an example of dissolution apparatus having 8 vessels and you have to run sample on hplc than collects from 8 vessels samples in one place(pooling) filter it and run it on hplc it is a pooled sample.
REGARDS
Amir
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