3D model in Cupcarbon, is it possible?

Dear colleagues, I need to create WSN model in 3 dimensions. I tried to use Cupcarbon for it but I couldn't find this function there. Could you explain me, how to do it in the package? Or to...

08 November 2018 1,481 1 View

What are your opinions about perspectives of Moebius strip like pathology diagnosis and treatment?

Proposed and tested an algorithm for diagnosis of Moebius strip like pathology, as prerequisite for treatment correction. The algorithm is reduced to initialization of study objects as a...

12 October 2018 5,854 3 View

Where can I physically get a large library of protein genes?

I am studying massively parallel protein-protein interaction. Is there a good source for a ready DNA library, where a protein gene (or part of it) would be surrounded by standard adapters? Like...

05 July 2018 2,909 1 View

fluorescent voltage sensors use?

Dear friends, i have a question regarding the fluorescent voltage sensors. I never tried it before, so I need good advice. What is your experience with Voltage dyes? If would use the...

11 January 2018 5,515 2 View

Do you have a job opportunity in Blockchain area?

Dear colleagues, My current project will finish this December and I'm looking for a new job opportunity in a Blockchain researching area. Write me please if you have an open positions for...

17 December 2017 4,894 3 View

How can I calculate using of energy by WSN node?

Could you write a link where I can find formulas for calculation an energy which is used by node of WSN (for transfer a data)?

17 December 2017 7,683 14 View

What problems are there for implementation blockchain technology in IoT, WSN and mobile devices?

All know that there are a problem POW for small devices. What with what problems have you encountered when implementing blockchain for small devices? What criteria do you use for evaluate new...

12 December 2017 9,647 6 View

What turbulence model and parameters to set on inlet of sintered/porous plate?

08 February 2017 4,939 3 View

Hello to everyone! Somebody knows where I can order Dermacentor reticulatus for laboratory culture?

I need a contacts!

15 September 2016 4,971 3 View

Is there a process of polynucleotide synthesis from nucleoside monophosphate, using energy from external chemical reaction?

Normally, DNA and RNA polymerization is realized by polymerases, using nucleoside triphosphates. The energy for the reaction is obtained from chopping off diphosphate group. I'm curious: is there...

15 December 2014 9,139 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

How many of absolute errors (in kcal) do you believe affect the prescription of the diet when estimating the energy required for an elite athlete?

Dear colleagues, I am writing an article that compares the measured and predicted resting metabolic rate in elite athletes. However, to define a cutoff point to determine which equation is more...

16 February 2021 4,515 3 View

Macrophages in culture not growing/ dying or detaching . Why ?

Hello, I am an undergraduate student doing my dissertation, where I isolate PBMCs from leucocyte cones in order to plate monocytes and allow for macrophage differentiation. Although the numbers...

09 February 2021 5,334 8 View

Rat Macrophage Markers for Flow Cytometry?

Hello, I'm trying to identify good putative macrophage markers for flow cytometry in rats.

04 February 2021 3,913 4 View

Can anyone help me with doing resorption pit assay on biomaterials for 28 days?

I culture RAW 264.7 cells on my samples and differentiate them to osteoclasts with RANKL. I want to do resorption pit assay by culturing cells for 28 days. My problem is that the macrophages keep...

01 February 2021 6,746 2 View

What M1 and M2 markers can I use for flow cytometry ?

Hi, I'm a student and I'm doing an internship. I need to asses the M1 and M2 populations of a culture using flow cytometry. From my researches, I know that this is subject to some controversy, as...

26 January 2021 299 3 View

What are the validation parameters for RAW BLUE macrophages 264.7?

Currently, I am working with RAW BLUE cell lines to check the SEAP expression on addition of TLR ligands? Method developed for different TLR ligands. How experiments can design for validations?...

24 January 2021 589 2 View

Can I use 24 wells black tissue culture uncoated plate for cultuing macrophage (THP-1 treated with PMA) ?or I have to coat them?

Hello, I will start an experiment using macrophage (THP-1 treated with PMA). I have ordered the plate that suitable for fluorescence plate reader which is glass 24 wells black uncoated tissue...

05 January 2021 4,214 3 View

No title

Hi, I am interested to see the effect of BCG on macrophages (microglia )Therefore , I would like to know the amounts and the routes of administration which is safe in mice and how long is...

20 December 2020 7,166 2 View