Are there differences between performance of two identical Simple Wes machines?

03 December 2020 8,975 4 View

Quick way to collect saliva to study viral RNA expression?

Hello, I am going to perform some saliva's RNA analysis. I found in the litterature several protocols to collect and prepare saliva for RNA/DNA analysis. For main of them we have to use kits and...

24 September 2020 4,937 4 View

How do I produce a carbon composite consisting of 2 metals?

I have a question in regards to the formation of a carbon composite for wastewater application. I came across some articles that suggest doing co-precipitaition, however those articles requires...

20 September 2020 9,793 5 View

How do I fix my fraction collector, Frac-920 and AktaPurifier?

Hello all, I frequently use the Akta purifier for protein purification and recently have been getting error messages in unicorn saying "error 2012 no response frac, 900". The frac 920 is designed...

02 September 2020 2,757 1 View

Questions pertaining the use of Graphene from sugar: How effective is the coating?

Hi scientific community, I have read some articles pertaining to the use of regular sugar to form graphene composites would like to know some questions pertaining to it Is the composite...

07 August 2020 4,719 3 View

What have been the effects of Covid-19 on digital engagement in the Heritage industry?

For my dissertation i am looking at digital history and digital engagement and how this has been effected by the Covid-19 pandemic. Any help with this research would be greatly appreciated.

29 July 2020 2,808 3 View

Why my PCR amplicons no longer have the expected size and are faint?

Dear all, I have issues with my PCR amplicons. For one week now, I do no longer have the expected size for my PCR amplicon (252 bp in the first 4 wells) on the 50 bp DNA ladder. They appear faint...

17 July 2020 4,988 5 View

CSTR in series: why is it that 2 CSTR is series performs better then 3?

Hi all, I having some issues with the performance of my reactors. some context to question: 3 CSTR is series All of the reactors are equal in volume Reaction follows first order. (Fenton-based...

13 April 2020 7,459 3 View

Papers on Hop Domestication?

Hello, I am interested in learning about the natural history and domestication of Humulus lupulus. I have been unable to find any papers on this subject, so I am wondering if anybody could...

22 March 2020 4,157 5 View

No color in hot water extract after successive extraction with n-hexane and methanol?

Greetings I'm doing an increasing polarity successive extraction on tree leaves with n-hexane, methanol, and hot water successively. The n-hexane extract gave dark green coloration, while the...

04 March 2020 8,687 3 View

Hello fellow researcher .. What is the meaning of your name?

I would like to create a small guide containing the meanings of the names .. Thanks to everyone who cooperates with me in achieving it.

03 March 2021 5,266 4 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

In Molecular Dynamics Simulation, how do I make changes to the force field according to my protein?

Hi In Molecular Dynamics Simulation, how do I make changes to the force field according to my protein? I got this error in the add-ion stage: Fatal error "./ amber99sb-ildn-HHH.ff / force...

01 March 2021 8,747 4 View

Does any one can help me choose a reasonable basis set for intermediate metal ions interactions with guanine (i.e Fe2+, Mn2+, Cu2+ and exc.)?

Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...

01 March 2021 6,187 2 View

Can polymer concentrations be calculated based on the number average molecular weight?

Hello, I spin-cast two different polymers onto wafers and measure their height as a function of initial concentration. For polymer A, I have the weight-average molecular weight (Mw) as well as...

28 February 2021 7,882 2 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

How can I publish a manuscript in free or low cost journal with good H-Index?

Dear all I am going to publish my manuscript which is from my thesis in a good H-Index journal but I found them very expensive(I don't have financial support) based on the exchange currency of my...

28 February 2021 7,800 6 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View