At this time we are facing challenge to treat most of infectious disease with commonly available antibiotics/drugs. In the conventional bacteriological procedure usually it takes a minimum of 48-72 hrs to cultivate pathogens, identify, and to check for AST. This procedure is actually time consuming and costly plus it may not help patients under emergency conditions. So, do any one share experiences or investigations under way on any any kind of point of care AST screening technologies to date?
Thanks,
Automated microscopic imaging, real-time PCR, mass spectrometry, microarrays, microfluidics, bacterial cell lysis detection, RNA sequencing, whole-genome sequencing, electronic nose devices, vibrational- and absorption-based spectroscopies, fluorescence-activated cell sorting, bacterial cell weighing by vibrating cantilevers, micro-calorimetry, optical tweezers, rotating magnets, . . .
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Article Rapid Clinical Bacteriology and Its Future Impact
You can use VITEK-2 rapid identification systems that determine bacterial antimicrobial activity by MIC test about 24 hour.
Thanks Muhammed Duman. We use the Vitek and its pretty quick for emergency purposes. though the molecular methods such as whole genome sequencing will be more rapid answering all sorts of clinical questions in one goal but as for Middle and low income countries it will take ages to have them around
If you want instant information about the organism can get by multiplex PCR system. Direct sample can be put into. Only important point is that you must be absolute sure about zero contamination, otherwise false result would be there. There are multiple systems over there. If you want answer from culture plates, Vitek and MALDI-TOF will help you to cut short time. For carbapenem resistance, PCR system is available to detect carbapenem resistance genes irrespective of bacterial species. This will reduce the time to administer appropriate antibiotics.
First, I have to say that empirical treatment therapy based on retrospective AST data and knowledge of prevalent bacteria within the area should help with initial antimicrobial selection for treatment.Be that as it may, I will suggest Vitek and MALDI-TOF as these techniques will reduce the waiting time.
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