has anyone had to deal with cell lines that proliferate at 33 degrees and differentiate at 37-38 degrees (14 days needed) celcius?
The cell line im dealing with are SVI podocytes (CLS). i initially tried differentiation induction at 37 degrees but no differentiation was observed. i then tried temperature at 38.5 degrees but i see lots of cell death, and as at day 10 of differentiation, only a small portion of the cells look dfiferentiated.
i have heard that podocytes cell lines are extremely sensitive, for example: if any of the cells were to be overgrowned previously, these cells would lose its property and become resistant to differentiation.
any advise would be greatly welcomed!
As you know, podocytes consist of the glomerulus, so that you have to perform co-culture.
what differentiation media did you use? it should not contain any interferon gamma because SV40 is driven by it.