Hi Colleagues,
Before digging into the literature and trying different approaches I want to ask the experts in here!
Regarding the freeze-drying process for PLGA NPs stabilized with different surfactants, what are the best conditions? my freeze-dried particles are not happy and aggregate up to 1400nm (initial size is 200nm)
will address some of my concerns if you could please help
- Snap freezing, I do freeze the particles on one go with liquid nitrogen.
- would using sugars as cryoprotectants cause minimizing the dispersing effect of the surfactant. I want the particles to be rapidly dispersed in hydrofluoroalkane solvent HFA 134a and I am making them in a way to be fully covered in HFA soluble surfactant to ensure well dispersity.
Many thanks!