we are evaluating the effect of natural products on wound healing in mice.
We are trying to design the multifunctional bioplymeric matrixes with fluorescent properties.
06 July 2018 8,218 3 View
We have checked ROS and mitochondrial membrane potentials integrity but need one more experiment to see the effects of our nanoparticles on mitochondrial ROS.
06 July 2018 6,946 1 View
We have done an invivo assay of the naoparticles that could target tumor tissues. How we can demonstrate targeting efficacy in vivo?
06 July 2018 3,652 1 View
We have synthesized a hydrogel matrix in which our drug is encapsulated.But the drug release is not satisfactory.Can anyone suggest how to increase this release efficacy?
06 July 2018 1,809 4 View
We want to evaluate the biosafety of the nanoparticles from biochemical biomarkers' detection results, for example, ASN, AST, UCN, etc in invivo system.Suggest some protocols to do this.
06 July 2018 7,799 0 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View
