we are evaluating the effect of natural products on wound healing in mice.
We are trying to design the multifunctional bioplymeric matrixes with fluorescent properties.
06 July 2018 8,101 3 View
We have checked ROS and mitochondrial membrane potentials integrity but need one more experiment to see the effects of our nanoparticles on mitochondrial ROS.
06 July 2018 6,812 1 View
We have synthesized a hydrogel matrix in which our drug is encapsulated.But the drug release is not satisfactory.Can anyone suggest how to increase this release efficacy?
06 July 2018 1,689 4 View
We want to evaluate the biosafety of the nanoparticles from biochemical biomarkers' detection results, for example, ASN, AST, UCN, etc in invivo system.Suggest some protocols to do this.
06 July 2018 7,681 0 View
We have done an invivo assay of the naoparticles that could target tumor tissues. How we can demonstrate targeting efficacy in vivo?
06 July 2018 3,532 1 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...
01 March 2021 6,953 1 View
Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...
01 March 2021 9,938 3 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...
25 February 2021 155 2 View
Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...
24 February 2021 1,825 3 View
Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...
24 February 2021 5,759 3 View
Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...
23 February 2021 7,701 2 View
Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...
23 February 2021 5,476 4 View
Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha
22 February 2021 3,891 2 View
