Induced pluripotent stem cells (iPSCs) hold great promise for regenerative medicine and disease modeling. The original methods to grow human iPSCs utilized methods developed for human embryonic cells (ESCs), in which mitotically inactivated mouse-derived fibroblasts are mainly used as a “feeder” cell layer to maintain the undifferentiated status of pluripotent stem cells.
https://www.thermofisher.com/kr/ko/home/references/protocols/cell-culture/stem-cell-protocols/ipsc-protocols/ipsc-cells-with-ksr.html (Thermo Fisher Scientific company have feeder free iPSCs but only for research purpose.)
My question is;
How to buy feeder-free iPSC cell which we can buy and use for business purpose (DIAGNOSTIC USE) ?
Dear Muhammad,
I am not sure if I understand your question, but,
Pluripotent stem cells (ESCs and iPSCs) can be cultured in feeder free commercially available feeder-free ready to use culture media. For instance, Essential 8 medium form Thermo fisher. The phrase "research use only" indicates that this product is not suitable for clinical application. You can order it because you are going to do some in vitro (diagnostic) tests.
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