Please tell me a good pipline to analyze human gut microbiome from 16srRNA based NGS data?

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Abhijeet Singh

You can use either local command line based analysis pipeline with DADA2* or QIIME2* /Mothur OR can use cloud based analysis platform from galaxy server (https://usegalaxy.org/) or MG-RAST (https://www.mg-rast.org/). For all of the pipeline, extensive tutorials and documentations are available over the internet.

(* preferred )

Harishchander Anandaram

Thanks Abijith.

Abijith Can you brief me the steps involved in Data Analysis from Quality Control using fastq files to identify microbiome with different OTUs.