Samiksha Kukal

6 Questions 2 Answers 0 Followers

Questions related from Samiksha Kukal

SiRNA mediated knockdown is observed at RNA level but not at the protein level. What might be the reason?

I am currently doing siRNA experiments and getting 80% knockdown at RNA level, however no effect is seen at the protein level even at higher concentrations tested. What more should i try?

05 August 2019 9,664 1 View

Please suggest me an inducer of PPAR alpha protein expression?

I am in a need of molecule which induces the protein expression of PPARalpha gene. Please suggest.

08 January 2019 7,431 2 View

Can someone tell me estradiol inducible gene in brain endothelial cell lines?

I want to know a gene strongly altered by 17 beta estradiol in human brain endothelial cells, so that i can take that gene as a control for the system.

02 May 2017 7,505 3 View

For how long we can store the processed microarray chips?

For how long we can store the processed microarray chips before scanning

17 October 2016 3,981 3 View

In microarray experiment on cell lines, should we take the technical replicate or biological replicate?

In microarray experiment on cell lines, should we take the technical replicate(loading sample from same passage number 3 times) or biological replicate (loading sample from cells of 3 different...

12 February 2016 821 4 View

How to decide time points of RNA extraction in microarray experiment?

Hello Experts, How do we decide time points of RNA extraction after giving drug to the cell lines for checking the gene expression profile in a microarray?

10 January 2016 8,790 5 View