Madhurima Gupta

6 Questions 4 Answers 0 Followers

Questions related from Madhurima Gupta

Native vs SDS PAGE electrophoresis?

I performed NATIVE gel electrophoresis and SDS PAGE electrophoresis for my porcine skin tissue ECM extracts. interestingly, I saw higher molecular weight protein bands in the case of SDS PAGE but...

29 August 2024 9,350 3 View

Can I use 1 M tris HCl buffer instead of 1.5 M in SDS-PAGE resolving gel?

I don't have 1.5M tris HCl buffer for the resolving gel but I have 1 M tris HCl buffer with pH 8.8 will that work for SDS PAGE? do I need to change the amount added to the mix?

12 June 2024 5,810 3 View

Characterisation test for proteins extracted in an acedic media?

i am working with lyophilised porcine skin. what tests can be carried out on the extracted sample to determine what proteins/ components are present?

09 February 2024 9,168 0 View

Please share lowry assay protocol for protein estimation?

please share an easy-to-follow protocol of lowry assay, along with the method to prepare BSA standard graph in the range of 5ug/ ml to 100ug/ml

17 August 2023 8,611 0 View

Method for protein extraction from porcine skin?

hi, I am working on extracting proteins from pig skin. Initially, I used plain 0.5 M acetic acid solution for extracting proteins and used the solution as such post-centrifugation. but the protein...

01 August 2023 5,771 3 View

How to determine the end point while defatting pig skin using surfactant?

I am working with protein extraction using porcine skin. prior to protein extraction I need to de-fat the tissue. I am using triton x to de-fat the tissue... how to determine if the defatting is...

20 July 2023 9,956 4 View