Dear Dhurgham Alhasan 

It is misleading, its better to diagnose by biotechnological tools, since colony forms vary when a mycoviruses are present

Pls. find the attached files

Hoping this will be helpful to your project

Best regards

Prof. Huda Kawas

http://www.asmscience.org/content/concept/Entity/ASM/Microbiology/Agricultural_Microbiology/Agricultural_Plant_Microorganisms/Plant_Pathogens/Plant_Pathogenic_Fungi

http://onlinelibrary.wiley.com/doi/10.1002/9780470278376.refs/pdf

https://www.science.gov/topicpages/g/genus+fusarium+includes

http://www.isppweb.org/abstracts/interno_icpp_2013.pdf

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=8&cad=rja&uact=8&ved=0ahUKEwi9hbaT_7fTAhWiFJoKHfllACwQFghTMAc&url=https%3A%2F%2Fscholar.cu.edu.eg%2F%3Fq%3Dhanem%2Ffiles%2Fmonograph_on_the_genus_fusarium.pdf&usg=AFQjCNFzg20Gyl78PxCowVS9PY__6BRmUA

http://www.mycobank.org/Biolomics.aspx?Table=Mycobank&MycoBankNr_=231142

Chapter Mycoviruses: A Novel Option for Managing Some Plant Diseases

Also

Hoping this will be helpful to your project

https://www.ncbi.nlm.nih.gov/pubmed/23498910

http://www.ndrs.org.uk/article.php?id=008011

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3892584/

https://www.ncbi.nlm.nih.gov/pubmed/24890361

http://apsjournals.apsnet.org/doi/full/10.1094/PDIS-05-15-0550-PDN

Dear Houda Kawas

Thank you very much for the help, I sent the fungus into Germany, and they did sequencing of ITS, and bioinformatics, however, they identified it as F.equiseti but this fungus morphologically formed the microconidia while in the literature reviews it can not form these conidia, so when I put its sequence within the BLAST appeared multi choices including F.incarnatuam and F. equiseti. Therefore I posted my question to confirm the identification. I repeat my thanks for you.

Dear Viji Viji

Thank you for help. The fungus had the following appearances:

Czapex Dox Medium

Whitish-gray with a pink center=Colony 

 Gray with  yellowish-pink center=Inverted colony

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MEA

White with concavely center=Colony 

 Yellow, especially in the center, was a dark=Inverted colony

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PDA

White but the center and edges were pink=Colony 

 Yellow especially in the center=Inverted colony

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SDA

Pink=Colony 

Yellowish pink=Inverted colony

-

You most welcome

Best of luck

Houda

Dear Dhurgham

It is difficult to diagnose Fusarium incarnatum according to the morphology of the colony. Since the shape of fungal colony could be changed even with the types of single carbon substrates. I attached Phenotypic characteristics, possibly helpful for identification of this fungus. Good luck

Dear Jawad

Many thanks for your words.

Dhurgham

Add an answer

Dear Dhurgham,

Fusarium incarnatum-equiseti is considered a species complex (FIESC). I strongly suggest approaching Dr. Kerry O'Donnell who is an expert in Fusarium taxonomy to clarify further and accurately identify your Fusarium!!

You can reach Dr. O'Donnell who is Senior Research Scientist at the following e-mail address: [email protected]

https://www.ars.usda.gov/midwest-area/peoria-il/national-center-for-agricultural-utilization-research/mycotoxin-prevention-and-applied-microbiology-research/people/kerry-o-donnell/

Microbial Genomics Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Peoria, Illinois, USA.

Best wishes and regards to you and beloved people of Di Qar!!

Dear Simon

I'm grateful for your help and thank you for serving the science and life.Yes, I used a sequence of ITS through using NCBI, and results appeared the different species including F. incarnatum and F. equiseti, so I posted this answer.

Dhurgham