Too low protein concentration for Western Blot - any advice for polyacrylamide gel method?

Currently have punched mouse brain samples of different regions that are of too low protein concentration for Western Blot. Has anyone used the polyacrylamide gel electrophoresis method to detect...

09 January 2021 3,955 3 View

Western Blot: any reasonable alternatives or troubleshooting advice for punched samples?

Hi, I currently have punched mouse brain tissue separated by brain region (using the cryostat) and the initial plan was to do Western Blot analysis on the punched tissue. We already have the...

01 January 2021 8,884 1 View

Does anyone have experience or suggestions on the polyacrylamide gel method of protein purification and measurement for punched samples?

Hello, I currently have sliced and punched mouse brain tissue using the cryostat and I wish to analyze it for the concentrations of different dopamine receptors. We initially desired to use...

01 January 2021 9,455 2 View

How to insert a line of median into scatter plot in SPSS? Multiple variables possible?

Hi! I am using SPSS chart builder to do grouped scatter plots. In the first scatter, I have two groups on the x-axis. I want to add a line of median to each group for better comparison. How can I...

17 November 2020 8,693 3 View

Economic evaluation in mental health services / DataBASE to use?

Hi! I am conducting a master thesis in the field of economic evaluation and mental health services as systematic literature review. Now I consider what databases to use. I have been thinking to...

09 September 2020 2,010 3 View

Can I use in a meta analysis case reports and rcts? And what calculation Do you raccomend( proportion, relative risk....)?

21 August 2020 5,190 3 View

Where can I find credible statistics for social media platforms?

I wonder where I can find credible statistics about patterns of using social networking sites that can be used as a reference in an academic thesis, preferably downloadable. Thanks

08 May 2020 4,932 5 View

Where can I find research papers or data concerning Demographic and Socioeconomic Characteristics of Immigrants and Refugees ?

Hello Everyone, I do hope that all of you are doing well and staying safe amid the COVID-19. I'm trying to find research papers or data that gives a global view about the difference in the...

03 April 2020 4,157 1 View

Why can't I see collagen in SDS-PAGE?

I try to resolve collagen on SDS-PAGE. My goal is to resolve the collagen extracted from skin scaffolds. I use ThermoFisher Bolt mini gels. I can see the protein ladder migrating and also I can...

15 March 2020 9,906 8 View

Your opinion on pulsed field electrophoresis instruments?

Hello, I am interested in running an experiment requiring pulsed field electrophoresis. The primary samples will be budding yeasts chromosomes and minor set of samples with chromosome sizes up...

30 October 2019 7,068 2 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What is the status of bread making and more bakery foods with the use of insect flour/powder?

Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...

03 March 2021 6,223 5 View

Any references about insects or animals that can damage or weathering of rock or fossil material?

Are there any references to insects or animals that can cause damage or weathering to the rock or fossil material? books or paper that would be nice. Thank you very much!

24 February 2021 6,805 3 View

Please help in identification of this insect?

This insect is minute in size around 1 mm and not clearly visible with naked eye.

22 February 2021 5,907 11 View

How reliable is estimation of copy numbers by absolute qPCR?

Hello, I would like to compare the expression levels of several genes in insects, so I prepared standard curves and estimated copy numbers of each sequence in my samples. But for one of the genes,...

11 February 2021 8,421 13 View

Do you know non-feeding larval instars in insects?

Hi, I am looking for information about insects having a larval instar that does not feed at all. In other words, able to molt to the next instar without acquiring any nutrient. Does anyone know...

20 January 2021 9,387 19 View

Triggering feeding of beetles / how to make them hungry?

Hey, I am working with Psylliodes chrysocephala feeding on oilseed rape. In feeding trials I often have the problem that a huge portion of the beetles do not feed at all. In clip cages I observed...

19 January 2021 9,851 6 View

Which sampling techniques can be used for huge flying insects?

Tropidacris collaris is a grasshopper. Adults, which can reach more than 15 of length, are strong flyers and actually they resemble small birds or bats. Most of the time T. collaris is found in...

13 January 2021 3,735 5 View

Can anyone recommend utilizing the plasmid extraction kit for extraction of the circular DNA genome of viruses associated with insect vectors?

Dear Colleagues, I am planning to detect and discover DNA viruses particularly those with a circular genome and transmitted by insect vectors. However, I am confused about selecting the best...

10 January 2021 9,534 4 View

What is the difference between "pesticides" and "insecticides"? Are they same?

What is the difference between "pesticides" and "insecticides" in English? Are they the equivalent replacement when used in text? I am an ecological researcher who particularly concerns the...

04 January 2021 6,230 37 View