Precipitate protein with organic solvents. Is it too low for silver staining?

There are a range of options, each has potential problems.

Freeze drying is typically the best from the perspective of preserving activity. It takes time though.

Centrifugal concentration / evaporation is relatively fast, but concentrates the salts

Membranes (spin type) are good but you can lose protein due to non-specific binding to the membrane, or size depending, you may see gel formation at the membrane, which gives you (activity) loss.

Kind regards,

Rob

One of the very simple, practical , and cheap methods is using spin concentrators. They contain a filter that will allow water to pass but not your protein. But, check if your protein Mwt is within the filter range (mostly it will be ). Store it 25 % ethanol to avoid contamination.

Spin concentrators are not cheap at all. Shelf life also sucks

You may use dialysis membranes. I think this is the cheapest way. An other option is precipitation of the proteins. You can use ammonium sulfate foprotein precipitation.

Thanks to you all. I have a whole wide range of options now

Thanks so much

Put your sample in dialysis tube and close it carefully after that put dry scroce sucrose and change that wet sucrose after 10 minute and contionouse change that wet sucrose until geat high concentration protein.