Origin and time-line of PCR use, it's application, limitations and shortcomings proposed by critics
Is this a school homework? The references on this topic abound freely on the internet.
Mr. Zakka, have a read of the following:
http://siarchives.si.edu/research/videohistory_catalog9577.html
Article Biochemistry, Polymerase Chain Reaction (PCR)
Article A Short History of the Polymerase Chain Reaction
The Taq enzyme belongs to termophilic Archaea (thermus aquaticus), as it is indicated in interesanting recommendation from Zakka. Something important in relation to this enzyme is that after 500 nt, it begins to make mistakes, that is why it is not used for procedures as site directed mutagenesis, where the fidelity of nucleotides is important. Others polymerases have been developed over the years for mutagenesis. There are also kits to introduce mutants, where less time is invested than traditional protocols. Taq can be used for amplify DNA fragments, it woks well, but procedures beyond a PCR, I would prefer to use another.
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