Please can Sephacel be used for trypanosomes elution from whole blood instead of DE52? Also, what column size is best?
I have attempted various methods, including homozygous gene disruption and CRISPR-Cas9, but have not achieved the desired results. It appears that the gene of interest is situated in a...
15 July 2024 3,977 1 View
Would an average spot welder suffice for welding tabs for pouch cell electrodes instead of an ultrasonic one? Why would an ultrasonic welder be better than a regular spot welder?
10 July 2024 8,763 0 View
The impact im looking for could be both positive and negative on the electric grid
18 June 2024 9,269 3 View
IT
09 June 2024 735 1 View
What are the major ethical issues to be considered while working on design and construction of electronics device in a research work.
03 June 2024 8,189 4 View
The impact of monetary policy and bank rate on the performance of deposit money banks in Nigeria
31 May 2024 7,587 1 View
The result from PlasmidFinder shows that the assembled genome harbored many plasmids. Consequently, I want to investigate whether the plasmids are encoded for ARGs. And determine if they are on...
08 May 2024 7,467 0 View
ls it possible to emerge the wi-fi and Li-fi in one device and regular them.
21 April 2024 9,292 0 View
The code needs to be able to work on a 28-bus system having cost as the objective function and constrain like transmission loss.
18 April 2024 3,349 1 View
I want to understand why most analyses conducted in Africa use GMM instead of other panel data estimation techniques.
16 April 2024 5,322 3 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
31 July 2024 2,150 0 View
Hi all, My lab has Thermo Scientific™ Invitrogen™ EVOS™ FL Auto 2 Imaging System, and I was wondering if I will be able to use it with whole blood, whilst focusing on platelets? The idea would...
24 July 2024 9,337 3 View
Hello, good morning. I would like to know which plate I can use to infect fresh PBMC with virus and culture for 24 hours. I have tried using 12-well and 24-well plates, but many cells adhere and...
24 July 2024 2,158 2 View
hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...
21 July 2024 8,660 1 View
Hi, I need help in detaching adherent monocytes from well plates. I isolated the PBMCs from buffy coat and I did CD14+ positive selection to collect only the monocytes. I let them adhere on my...
20 July 2024 7,922 2 View
These datasets will be used in the training of machine learning algorithms. Does anyone know any available data?"
17 July 2024 6,519 3 View
Type 2 diabetes can be managed and, in some cases, put into remission through proper diet and exercise, regardless of age. Here are some key points: Weight Management: Achieving and maintaining a...
07 July 2024 8,745 2 View
I'm simulating a magnetohydrodynamic problem of blood flow using lattice Boltzmann method in lattice units and I need to convert the magnetic conductivity to lattice units. The physical value is...
07 July 2024 5,243 0 View