Do blood cells die after staining in blood smear analysis ?

This might be a very naive question but I've not been successful in finding sources that explicitly say that the cells are alive post staining. Do Romanowsky stains such as Giemsa, Wright etc....

09 September 2020 2,920 3 View

How and where do I get a reflective essay reviewed and published?

I spent the past few months interning as an independent researcher at the Field Museum in Chicago. Here, I spent my time observing the design procedure that leads up to the creation of a...

23 May 2020 770 10 View

What is the lower limit for reliably counting cells in a typical hemocytometer ?

I am looking to quantify the counting errors associated with serial dilutions. Few sources mention a lower cap of 25,000 cells per ml - does this sound right ?

27 December 2019 3,714 4 View

How to design Model Predictive Control for Uncertain Nonlinear System with input delay?

Suppose we have a nonlinear system: x_dot=A x + B u(t-T) + phi(x) + d(t) y=Cx where x:state, u:control input, d:exogenous disturbance (bounded), phi(x): nonlinear vector (satisfy local...

24 June 2019 6,908 3 View

Can anyone answer the "Invalid cp problem at a particular temperature in fluent"? It will be a great help?

Invalid cp problem occurs in my fluent window. If I change cp of one material then same error occurs due to other. Is it a problem of mesh? Or any other problem. Please Help.

09 April 2019 1,977 3 View

Which AI platform is being used by Wireless Communication researchers for implementation of various ML/DL algorithms in their research?

I am more used to MATLAB, but I can't find suitable tutorials for advanced DL implementation in the context of Wireless Communications, using MATLAB. If you can suggest the answer, please let me...

21 March 2019 7,453 3 View

I have made a flexible i.e. less stiffness NFRPMC, How to achieve stiff composite?

Hello All, I have made a laminate which is very flexible which can be cut into parts by bare hands. I cured it at normal room temperature for day and half by placing load on laminate during...

08 June 2018 9,110 3 View

Are there studies on Indian climate history over the last 2000 years?

I'm attempting to build a hypothesis about the Gupta Empire of classical India, and am interested in understanding monsoon and temperature levels. Tree rings, Himalayan ice cores, any leads would...

18 March 2018 1,679 5 View

Can anyone inform me about some "rapid" peer-reviewed "SCI or SCI(E) indexed" journals in the field of Wireless Communications and related areas?

I need to publish some of my recent works in reputed journals but, unfortunately, I am short of time. So, I urgently need information about the aforementioned journals.

17 February 2018 1,985 4 View

Is there a specific model for gasification in fluent or do we have to use a UDF ?

I have performed combustion quite a few times but I am unable to do gasification. Is there a particular model in fluent or do we have to use a UDF. Do we specify products in species transport or...

21 January 2018 6,115 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View