Aditya Soni

6 Questions 6 Answers 0 Followers

Questions related from Aditya Soni

How to recover recombinant TGFB1 from leaf tissue extract if free for of TGFB1 is really hydrophobic and easily lost during purification?

hello All, I am trying to produce Human TGFB1 in tobacco by using Agrobacterium mediated transient expression. The expression is very good, protein is perfectly soluble in buffer. In the...

01 January 2019 4,891 0 View

Any recommended method to grow E.coli JM109 having a plasmid size of 20kb?

hello all, I have transformed a 20kb plasmid into E.coli JM109. But the problem is the growth time has become too high, may be more than 30 hrs and so. Colonies start appearing on plants after...

07 July 2018 7,608 6 View

Will Pig, Mouse specific Anti-GDF11 antibodies recognize human GDF11 in Western Blot?

I am working on human-GDF/BMP11. I ordered anti-GDF11 antibodies (poly-clonal) and its description says, Specific activity against Pig and Mouse. Will it detect human GDF11 in Western analysis?...

11 November 2017 8,169 3 View

Plasmid extraction, false yield?

Hello everyone, I know it is a very basic question. Whenever I extract plasmid by alkaline lysis method and take nanodrop reading, it comes around 03-05ug/ul. Nanodrop peak is at exactly 260nm,...

02 February 2017 2,710 4 View

"Intein Protein Tag", someone Knows?

Hello Everyone, I am trying to develop a protein expression and purification system. Hence, can anyone tell me about "Intein Tags" or related papers, articles or suggestion? Please Help Thanks

01 January 2017 8,776 3 View

His-Tagged Human TGFB1 purification from Nicotiana benthamiana

hello all, I am trying to produce His-Human-TGFB1 in tobacco leaf tissues using agrobacterium mediated transient expression system. The Purification is based on His-Tag Ni-NTA system. Protein is...

01 January 1970 269 3 View