Plant DNA extracted pellets are not easily getting dissolvde in TE buffer.
It could be due to over drying of pellet. After adding TE incubate in 37C for 10-15 min or till it dissolves
My pellet dissolve pretty easy in TE-Puffer. Wait some minutes and flip at the reaction tube! It will dissolve easily! Cheers, Nadine
It will be rehydrated at +4oC overnight in TE buffer. Moreover, if you diggest RNA with RNAse, you will need to incubate DNA solution at 37oC, and, as Mr Bhat says, it will be dissoved for sure.
Dear Shelat, It may be due to over drying of your pellet. You may store it at 4C overnight and flit it lightly in the morning.
Cheers!
Dear Shelat, you didn't said anything about the method you used and the plant from where you extracted the DNA. All the suggestions above are very useful but if the problems persists you should think on changing other things than just resupension method of your DNA like the extracion method. Tobacco DNA is normally quite easy to disolve but DNA from sweet potato for example, may be very difficult to get your DNA back in solution. If you are getting your DNA from one of those stubborn plants, the extraction method can make the difference. All the best and good luck!!!
I use to put the tubes one per one agains the tube rack and move them back and forward against the rack so the content of the tubes get mixed vigorously. I do it 5 times per tube. As soon as some DNA is dissolved you'll have enough for PCR. Good luck!
This problem is generally due to impure DNA. So you can just do purification step and get your pellet easily dissolved in TE buffer.
Secondly after adding TE buffer you can incubate it at 37 C for some time.
Generally this problem arises when u get whitish pellet instead of transparent pellet and the reason is pesence of impurities
Thank u Shubhneet Kaur.. but when i check OD the ratio of 260:280 shows 1.8.. but pellets taking very high time to get dissolve... Thanks Jorge.. Thanks Daniel... I am using General plant DNA extraction method for extraction of DNA from tomato plant leaves...
DearMargi
Just in case, I am sending you a protocol for plant DNA extraction. It is quite new an basically is an improved CTAB DNA extraction method.
I hope it will be useful for you
Dear Mr. Daniel Ducasse Thank u very much for protocol.. I am using the same protocol... and now i am getting very good bands on agarose gel...
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