Plant DNA extraction

More Shadi Alipoor's questions See All

Best novel method to optimize 3D geometry and its fluid?

Do you have any idea what is the best method to optimize 3D geometry and its fluid without linking the code with fluent (or any flow solver) ? In order to find the best 3d geometry and decrease...

10 April 2020 9,321 1 View

I have problem with cytotoxicity test of gemcitabine.how can i fix it?

Hi, i am working on effects of gemcitabine in HepG2 cell line and i should use the IC50 of Gemcitabine in the experiments.i found different values in literature and the numbers are highly variable...

06 December 2019 6,382 3 View

What is the IC50 of Gemcitabine in HepG2 cell line?

Hi, i am working on effects of gemcitabine in HepG2 cell line and i should use the IC50 of Gemcitabine in the experiments.i found different values in literature and the numbers are highly variable...

26 August 2019 8,545 4 View

In flow analysis in FLAC software, what is the reason for moving the model after the flow analysis in the earth dam?

Hello I have a model of earth dam in FLAC 2D and I did an uncoupled analysis for mechanical and flow. (set mech off/flow on ) Next step, when mech on/flow off ,the model starts to move in the...

14 July 2019 5,146 3 View

Do any researcher have ideas for the best medical Phantom that could be employed in segmating 3D medical data?

I am doing a research for segmenting 3D medical volumes using different techniques I used recently NEMA IEC body phantom and CIRS abdominal phantom,model 057 in my research I am looking for the...

19 May 2019 9,979 2 View

I have problem with designing a primer for my gene,how can i fix it???

Dear all.Hi. i want to design a primer for gene(BCl2) that have multiple variant. And I can not design a primer that includes all my gene variants and also be specific for my gene. what should i...

13 January 2019 2,430 2 View

How can i clone a plasmid?

how can i clone a plasmid that i don't know any thing about its sequence , and the backbone of the plasmid?

05 January 2019 6,028 15 View

Is degenerate primers appropriate for real time pcr?

hi... can I use degenerate primers for real-time? I have some genes related to the enzyme in the root but these genes are gene families .and I don"t know which gene controlled my desire enzyme,...

20 December 2018 3,452 2 View

Why do i have band in negative control in ITS primer?

I look for variation in strain bacterium and I want to do sequencing but I have a band in negative control that same in my strain bacterium first time I noticed band in my negative control, I...

02 December 2018 5,889 10 View

Hi, i am looking for anionic polyacrylamide or poly(acrylic acid) with charge density around 2 meq/g and low mw of 50000 g/mol?

what chemical suppliers are providing such polymers?

09 September 2018 7,331 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

Ali Nozari

1. Extraction of desired genomic DNA

2. Extraction method (CTAB with slight changes)

3. Delaputa's method

4. Khanujah method

5. Spectrophotometry

6. Polymerase (PCR)

And ...

Mohsin Swati

The results demonstrated that sodium acetate precipitation and oligo (dT) improved the quality of the extracted DNA significantly (p < 0.01). Also, sodium acetate precipitation, using oligo (dT), incubation at 70 °C and SDS treatment increased the quantity of DNA significantly (p < 0.01).