have you tried culturing microbes on any plant culture media?
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1. No, we grow plants on plant medium and grow bacteria on bacteria medium.
2. However, we do see some Agrobacterium can grow pretty well on MS medium (a plant tissue culture medium). Sometimes, it reaches the extent that no one can even eliminate them by using antibiotics.
Hi Elhussein,
1. I have read the paper you authored: "Plant powder teabags: A novel and practical approach to resolve culturability and diversity of rhizobacteria". It is interesting to see that simple plant materials can be used as a bacteria culture medium, and increase the 'culturability' of some 'un-culturable' bacteria.
2. One thing I am concerned: we all know that plant materials/extracts/exudates can affect bacterial behaviors, such as plant phenolic compounds can increase the virulence of Agrobacterium. Plant extracts can also affect bacterial global gene expressions (see attached paper), different plant-tissue extracts can cause different bacterial gene expression activities as well. If we culture these bacteria on medium consist of crude plant materials (such as leaves, without any minimum process or purification), it likely will change the gene expression profile of the bacteria and distort the experimental results of downstream experiments, especially when those bacteria are intended to be used for gene expression-related experiments later. What do you think?
Well, IMHO I think that if we grow bacteria on the same source of isolation, this will reflect/reveal the true gene expression profile, while on the other hand, growing bacteria on any media that is not identical to its natural habitat will alter that gene expression profile
tldr; gene expression profile of bacteria growing on plant culture media is the standard profile, otherwise it is an alternation.
Even to the bacteria associated to lichens!
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