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Hi! I'm doing pigment analysis (chlorophylls and carotenoids) of seaweed, and I have some questions about the extraction and the reference system. I'm not sure if I should use fresh or dry...
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Hi! We have made an experiment examining the effects of a compound on microbial growth using triplicates for each growth condition. For some of our data it's very clear that the compound had no...
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I’m working on extraction of secondary metabolites from fungi and considering using a mix of solvents with different polarities. Does mixing solvents increase the range of compounds that can be...
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If a protein sample is contaminated with nucleic acid, will these DNA/RNA run on the gel and show bands? (gradient gel, 4-20%, MW-marker: 14.4-116 kDa)
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Can DNA or RNA contamination in a protein sample affect SDS-PAGE results? Would it interfere with band clarity or migration?
06 December 2020 4,988 4 View
This is our first time doing SDS-PAGE, and therefore we would like to hear from more experienced people if we interpret the results right. Attached is a picture of one of our gels with our...
02 December 2020 1,670 3 View
I have run a SDS-PAGE with a protein molecular weight marker with a size range from 14.4 to 116 kDa. I get a band lying under 14,4 kDa. Is it possible to determine the size of proteins in this...
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