How do you measure the affinity of a secondary antibody to a primary antibody?

I have conjugated a dye to a goat anti-rabbit secondary antibody. I believe that the more dyes that I conjugate to the antibody, it then loses its ability to recognize the primary antibody. I...

18 June 2020 4,691 3 View

How do I remove an author who is not an author from a publication of mine?

An author got added to a publication I was uploading that is not an author on the article. How do I delete a name from the list of authors?

02 March 2020 2,110 5 View

What are the statistics of use for the automatically triggered, obligatory subjunctive in Romance, especially Spanish?

In the Romance languages, many syntactic contexts, especially in subordinate clauses, require a subjunctive mood on the verb, not leaving any romm for mood choice. In other contexts, however,...

16 February 2020 3,747 2 View

Does a statistical test exist that analyzes trends between experiments?

Hello! Here is a brief overview of my experiment and problem. I am doing Propidium Iodine staining on organotypic brain slice cultures to test effect of alcohol and inhibitors on cell death. We...

13 January 2019 9,159 3 View

How can i transform the plasmids pTargetF and pCas with electroporation into E. coli MG1655?

Hi! For my thesis I'm working on a CRISPR-Cas9 project, where I have to transform the plasmids pTargetF (2117 bp without N20 sequence https://www.addgene.org/62226/) and pCas (12.545 bp...

12 June 2018 1,834 10 View

HN2-5 cells dying after 4 days - why?

12 February 2017 6,626 2 View

Have someone recently studied lepidoptereans in canopies of Pinus?

19 April 2016 1,731 3 View

I am going to make a rat prostate homogenate to label TRPM8 by western blot. I dont know which sample buffer is the best, or if PBS is sufficient?

To standardize TRPM8 expression in spinal cord samples, we intend to first verify TRPM8 presence at prostate tissue (from rat). I would like to know what type of processing is needed in this...

07 October 2015 4,542 1 View

Can someone recommend some good conferences/ workshops on data analytics and big data in 2013?

I am looking for good conferences or workshops on the topic of data analytics and big data this year. Is there anything where the deadline for submission is not yet expired?

09 July 2013 9,193 8 View

I wonder whether anyone else has had any more success in obtaining a higher miRNA quality, or whether anyone could recommend any isolation protocols?

I'm currently working on the isolation of miRNA from human serum samples. Using the column based protocol, I have been able to isolate around 5ng/ul of miRNA, but the quality of RNA (260/280)...

19 June 2013 753 2 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How can we simulate an hyperelastic-viscoelastic behavor with Ansys APDL?

I am working on modeling and simulation of biomecanical material behaviour, I have succeded on simulating skin using anisotropic hyperelastic material, on APDL and in our lab's finite element...

28 February 2021 552 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Can ImageJ software be used to quantify collagen from Masson's-Trichrome stained images?

I am after quantifying the blue stained collagen in a Masson's-Trichrome stained skin section from an immune deficient mice and compared the same with its WT control. I am curious to know can...

17 February 2021 384 3 View

How to inter pain intensity data using NRS when patients report their pain in spectrum?

I have been working on a pain intensity study and I wanted my patients to report their pain intensity using numerical rating scale. many patients have reported thier pain in spectrum like 2 to 3...

13 February 2021 6,598 3 View

What statistical test is required for an analysis on pre-COVID & post-COVID patient improvement after attending a 10-week programme?

Hi all, Myself and my supervisor are looking at carrying out a statistical analysis to see whether there is a difference in patient outcomes for our 10-week pain management programme according to...

10 February 2021 6,622 10 View

I.P. drug injection- time to reach brain?

I am injecting different serotonergic drugs into mice i.p. and see an effect in the brain electrophysiology quite fast, i.e. within minutes, when using a specific receptor agonist drug. I do not...

01 February 2021 8,406 3 View

I'm looking for a dataset of eeg recorded during the pain stimulation. Anyone can help me to find it?

I'm looking for a dataset of eeg recorded during the pain stimulation (prefered the cold pressure pain stimulation). Anyone can help me to find it?

25 January 2021 301 2 View

Where can I find Raman Spectroscopy data set for breast cancer from blood plasma samples?

Where can I find the Raman Spectroscopy data set for breast cancer from blood plasma samples?

18 January 2021 8,192 2 View