Phylogenetic analysis of the gain/loss of genes along different species lineages?

More Ramanandan Prabhakaran's questions See All

According Bernoulli's theorem - Faster fluid moves with low pressure and slower fluid moves with high pressure?

Can I get brief explanation about this this theorem, which is applied in venturi meter ?

25 October 2023 2,438 9 View

Why there is precipitation after lipid extraction process ?

Hi, I have been working on lipid extraction from marine microalgae using hexane/ethanol as the solvent. I am facing precipitation issue during the final part of the lipid extraction, which is the...

07 May 2023 5,964 0 View

How to take 1.93 milliMolar from 58 milliMolar stock solution ?

Has per our reference we made 58 mM stock of reagent metol and for working we have to take 1.93 mM from stock, what is the method to take working or any dilution factor or formula available ?

23 November 2022 3,676 0 View

How to make final 3 ml volume with the given three agents ? volume of each reagent ?

A test was carried out in a final 3 ml reagent mixture containing 1.93 mM Metol, 68.6 lM copper and 1 mM acetic acid/sodium acetate buffer of pH 5.4.

12 November 2022 8,249 9 View

Proteinase K assay for assessing mitochondrial protein localization-what went wrong?

Hello everyone, I'm trying to perform Proteinase K assay for downstream analysis of the mitochondrial localization of a protein of interest. I isolated mitochondria from HeLa cells (using...

18 October 2022 2,106 4 View

My LFA has blood separation pads but Why Nitrocellulose is producing a red colored background when the sample is Blood?

I am working on developing a POC (lateral flow immunoassay) which uses blood as sample. The problem I face is that when the Test is initiated, the Nitrocellulose pad is having the blood particles...

12 April 2022 8,701 1 View

Can we measure the online runner wall thickness in pool type runner in blast furnace ?

In blast furnace ,pool type runner is used to reduce the frequent patch up of runner while furnace is in running condition.Whether any instrument is available to measure the runner wall thickness...

27 February 2022 6,154 2 View

Do any of my colleagues here in India possess a vibracorer/ know of a lab that does vibracoring in India?

I am looking for a simple vibracoring equipment for sampling wetlands, any leads from my friends and colleagues in India will be appreciated, thank you. Would be happy to import too.

21 December 2021 9,168 1 View

Thesis Topic Selection: Current Issues in Software Project Management?

Dear all, I am a student of Master of Business Administration and I have to submit a thesis for the completion my degree. I am trying to understand the current issues faced in the field of...

31 March 2020 752 4 View

What is an accurate method for measuring bandgap of quantum dots?

Can you suggest an accurate method for measuring the bandgap of solution synthesized quantum dots.

13 March 2019 8,833 5 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

António Amorim

I am not sure to understand your question, but first clarify if you mean genes (active) or sequences (pseudogenes and alike)

Ramanandan Prabhakaran

Thanks Dr.Antonio. I have updated the query. Please let me know about it.

Well - then it means you have (access to) a collection of reliable whole genomes for each of these strains/species. Is that so?