A test was carried out in a final 3 ml reagent mixture containing 1.93 mM Metol, 68.6 lM copper and 1 mM acetic acid/sodium acetate buffer of pH 5.4.
Make a larger batch (the reagents are inexpensive) and pipette out the 3 ml you need.
Thank you James Demers sir for your suggestion. Can you brief us about the volume of each sample to be prepared according to concentration (i.e.- 1.93 mM Metol, 68.6 lM copper and 1 mM acetic acid/sodium acetate buffer of pH 5.4).
You have 2 options: either you can prepare 100 mL of solution with the same concentrations and use only 3 mL or you can prepare 100 mL solution with 5 times concentrations and then dilute it 5 times until you get 20 mL of desired concentrations. Hope this helps
Is there any reason you can't just weigh out the metol and copper salt, and add acetate buffer to the appropriate volume?
There is no reason about it sir James Demers . your are saying like I should weigh metol (1.93) and copper salt (68.6) and to add in appropriate volume of buffer. Am I correct sir or any changes.
Thank you for suggestion Timur Ashirov sir. But we are having 3 reagents sir with that we have to makeup it to 3 ml solution. Is it possible with your suggestion method sir ?
That's all you need to do. Make up whatever volume is convenient, and use 3 ml at a time.
i) While considering an acetic acid / sodium acetate buffer solution:
The pKa of acetic acid is 4.75, from what we can only expect fair pH buffering effect between approx. 3.75 to 5.75. The pH of acetic acid / sodium acetate buffer solutions can be simply calculated based on the Henderson-Hasselbalch equation, after the molar concentrations of acetic acid (Ca) and sodium acetate salt (Cs) at the buffer solution: pH = pKa + log10(Cs/Ca); what can be generally expected to hold for Ca > 100Ka. A possible derivation for this equation, along with a justification for the stated minimum concentration limit for its validity, can be found elsewhere at this forum: https://www.researchgate.net/post/pH_calculation_of_a_mixture_of_formic_acid_NaOH_and_water
ii) Simply dissolve metol and the copper salt in above sol. according to the intended M conc. (i.e. mol solute / dm3 of the final solution containing both solutes, metol and the copper salt).
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