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Questions related from Nawaz Jia
I am trying to detect protein condensates at the endogenous level in tissue samples. I understand that IHC or immunostaining is the preferred method for this purpose. But just in case, can we use...
13 May 2025 3,179 0 View
I am trying to analyze protein protein interaction with wild type as well as mutated version. but alphafold 3 give interaction which i am not able to interpret like we just need to note PTM,iPTM...
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Hello, I am doing recombinant cloning. for this purpose i have to cut plasmid and then gel purify the right size band for further procedure. but in my case, i am using TIANGEN gel extraction kit...
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Hello, Does anybody have idea about any software or database that can give information about the possible phospho sites located in a protein? I am trying PhosIDP but its not working or loading...
16 July 2024 1,986 3 View
Hello, I am going to generate knock-in zebrafish model for my gene using Bi-FoRe plasmid. the gRNA target site for my gene has 43 percent efficiency. so i did microinjection using 600ng of Cas9...
09 July 2024 8,992 0 View
hey, I am designing gRNA target site to check the endogenous expression of protein in zebrafish. For this I am using chop-chop site. But most of the sites are in exonic region. I couldn't find...
14 April 2024 3,786 3 View
Hello everyone, This is my first time working with plasmids. My main goal is the expression and purification of proteins of interest. I am using a plasmid with CDS of another protein, which I need...
17 January 2024 4,970 7 View
