Phosphate solubilizing bacteria?

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What is the reason I get 400nm nanoparticles using an extruder with 200 nm membrane for cell membrane coating?

I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And...

30 July 2024 3,294 3 View

How Through Silicon Vias (TSV) simulated in Silvaco?

How can we simulate TSV in the Silvaco example? Exactly, I need the instructions for the attachments file. Best regards.

29 May 2024 1,638 1 View

How the ventilator generates positive pressure in PSV?

I read that "PSV assists the patient's effort by delivering a positive pressure during inspiration. This reduces the work required to expand the lungs and overcome airway resistance, making...

20 April 2024 1,660 1 View

How to increase the efficiency of doxorubicin entrapment in albumin nanocarrier?

I obtained up to 30% encapsulation efficiency by using the desolvation synthesis method, in different pH level(7-8/5) with 8% glutaraldehyde as a cross-linker, this work has been repeated many...

19 April 2024 8,496 3 View

Is rhodamine B can be used to prepare chlorophyll ‘a’ concentration?

I want to measure spectra of Chlorophyll ‘a’ in the lab , Is rhodamine B can be used to prepare chlorophyll ‘a’ concentration with the aim to measure spectra in lab using spectrophotometer?

14 April 2024 7,550 0 View

Why reflectance is greater than 1 in some cases?

I prepared different concentrations of chl-a using Rhodamine B (CHClN₂O₃) as indicator, my aim is to measure the spectra for each concentration trying to find relationship between them, the...

19 March 2024 2,147 0 View

what the applications of Tag SNPs selection and Haplotype Blocks are in the context of rare pathogenic variations?

Hi, Could somebody tell me what the applications of Tag SNPs selection and Haplotype Blocks are in the context of rare pathogenic variations in a Mendelian disease. Appreciate your...

17 March 2024 4,034 0 View

What are the best HW p-value & MAF cutoffs based on my study ?

Hello, Based on my research question, I would like not to exclude variations that are out of Hardy-Weinberg equilibrium, and I also want to have rare variants in my study ( i mean the MAF...

07 March 2024 5,788 0 View

How display the SNPs positions along a chromosome on the top of my LD plot which provided by Haploview?

Hi, I have provided my LD plot by Haploview and I used Linkage Format (.ped & .info) to this aim. I am wondering how could I have the gene structure on the top of my LD plot ? I mean I need...

06 March 2024 5,150 2 View

Is this a bacillus subtilis colony? on Hicrome bacillus agar medium?

I bought Bacillus subtilis ATCC 6633 bacteria, I grew them on Hichrome bacillus Agar media: green colonies with white crincle or wavy edges and the media changed from pink to yellowish. Is this...

01 February 2024 6,901 0 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...

05 August 2024 4,945 3 View

Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.

29 July 2024 8,560 0 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

Question about MHB (Mycorrhiza Helper Bacteria) isolation technique??

What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.

26 July 2024 7,387 0 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

How to prepare bacterial conditioned media to study the effect of bacterial secretome?

I would like to investigate the effect of bacterial secretome on cancer cells. How do you prepare conditioned media from bacteria?? conditions- time points - media I am looking for the best way...

17 July 2024 3,785 9 View

How do you decide an optimal AC amplitude for non-faradaic electrochemical impedance spectroscopy?

I work with planar interdigitated electrodes (IDEs). The finger width and the spacing between the fingers are 5 micron. The surface of the IDEs is coated by a non-conductive polymer layer...

16 July 2024 5,031 1 View

Can I use candle-jar technique to lactic acid bacteria?

I don't have the anaerobic generators (Anaeropac) so I'd like to try the candle-jar tecnique for the cultive of lactic acid bacteria

15 July 2024 8,890 2 View