We know that at a lower pH, after phenol chloroform extraction, RNA remains in the aqueous phase and DNA at the organic phase. But when we perform the same with Tris saturated phenol (Higher pH) DNA comes to the aqueous phase. Can anyone tell me the reason behind it?
Martin J Stoddart
This is also being discussed here
https://www.researchgate.net/post/Which_one_DEPC_TRIzol_Reagent_helps_RNA_to_be_in_supernatant_part_in_RNA_isolation_procedure_and_how
And to copy what I wrote there
Nucleic acid (mRNA, DNA, rRNA etc) will be in the aqueous phase at neutral pH. When Acidic, as is the case with TRIZOL, the double stranded nuclear DNA precipitates and ends up mostly in the interface.
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