Topoisomer of DNA - FAQS.TIPS

09 September 2011 1 836 Report

We all know ethidium bromide can intercalate DNA. My question is that which topoisomer (positively or negetively supercoilled) of DNA can take more quantity of ethidium bromide?

Amrita Ghosh

In case of negatively supercoiled DNA that will be higher. As we know negatively supercoiled DNA has a lower linking number value (Lk) as compared to positively supercoiled DNA, thus the previous topoisomerer will always be slightly relaxed, because of this negatively supercoiled DNA will take more EtBr

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