I have a project for drug screening with a phage display peptide library. To have a phage display peptide libraries, it may take a long time of construction.
Please give me the guidelines refer to the topics "Rapid method of constructing a phage display peptide library for research purpose?" or suggest me any other techniques to have a random peptide tools for drug screening
The challenge is the diversity. Look for the publications by Tomlinson and Winter on phage display (the I+J libraries) and possibly these papers: Article Advancement and applications of peptide phage display techno...
and https://www.mdpi.com/1422-0067/23/15/8554/pdf
There are many commercially available kits that have had good success. For example, randomized linear or disulfide-cyclic libraries are available here: https://international.neb.com/products/e8212-phd-c7c-phage-display-peptide-library-kit-v2
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