Hi there

it has been many years since I did IVT from plasmid but you usually have to linearise and purify to do IVT

That is because non linear plasmids have secondary structure that can prevent efficient IVT

Laurence Stuart Dawkins-Hall Thanks for the explanation. So, I can use both of them for linearization and then IVT?I mean It said SC is biologically-active form of plasmid, But for IVT ,I can linearize both of them and then doing IVT?

I would linearise both