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Hello I want to apply Biogel P-6 as a SEC resin. But I don't know how do its CIP. Last time, I used ethanol20% for CIP and its flowrate has decreased significantly to 0.5 ml/min due to very high...
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I use a column for purification. In its brochure it is said binding capacity is :0.3 mmol Ligand/ml resin and molecular weight of ligand is 340.4 g/mol. How can I calculate its binding capacity?...
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I extract plasmid DNA by cell lysis extraction method but the yield is so low in contrast with some commercial kits like Quigene or GeneAll. What are possible reasons?
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I want to perform an IVT by plasmid. It is necessary to use SC form than OC form or I can use both forms for linearization brfore IVT?
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