Peptide/Protein conjugation? How do you decide the ratios of peptide and protein if you want to conjugate them?

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How can we use mobile apps for improving students' academic performance?

Mobile apps can be a powerful tool for enhancing academic performance, how can we use mobile apps for improving academic performance

04 August 2024 9,492 0 View

How to calculate tumor size estimated doubling time when the initial volume is zero?

I am writing a case report on a rapidly growing symptomatic brain tumor. The patient had an initial brain MRI which was unremarkable, and no brain tumor identified. after 47 days the patient was...

26 April 2024 6,861 0 View

How do I get a satellite image from Ikonos Satellite for the year 2023/2024?

How do I get a satellite image from Ikonos Satellite for the year 2023. 2024

19 April 2024 3,146 0 View

How to separate nanoparticles from solution?

Hi everybody, I want to separate nanoparticles from a thick solution. I used centrifuge 4000 RPM for 60 minutes three times, but it stands sill. Could you suggest a better way, please? thank you...

07 February 2024 9,113 3 View

How can I download raw MS-based proteomics data?

Proteomics

19 January 2024 1,802 1 View

How can i test the dissolution of Doxorubicin after loading it on the nano carrier?

Nanoparticles

09 January 2024 2,696 1 View

Article How to place an article on the website from the magazine's website itself?

How to place an article on the website from the magazine's website itself

19 November 2023 9,718 2 View

What are the recent most important topics for research now adays?

Suggest for me topics I can write about

19 October 2023 9,191 4 View

Shannon's Formula Parameters?

Hello researchers, I've encountered a problem while utilizing Shannon's formula to calculate the transmission rate from various sources. It appears that the transmission rate is highly sensitive...

30 September 2023 8,593 4 View

Is it possible to create an algorithm or programming language ?

that is flexible and self-learning capable of automatically generating the right code to do.

23 July 2023 4,899 10 View

How to increase simulation box size?

We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...

08 August 2024 4,842 0 View

Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,218 4 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How to analyze multiple phosphorilation sites?

Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...

04 August 2024 8,432 3 View

Recovery rate after peptide desalting using Sep-pack C18 1 cc Vac Cartridge?

Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?

31 July 2024 6,544 11 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Conjugation of PEG-Amine to an Amino Acid Using EDC?

I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...

31 July 2024 2,033 1 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View