needing to detect peppermint oil mixture with Budesonide in HPLC, C 18 column due to overlapping in UV-VIS spectroscopy.
Can I know the Mobile Phase and Diluent which you have in your present method for HPLC?
Only after that I can help.
Or are you asking for a new method?
the mobile phase is methanol : water ( 80:20) and diluent is methanol 100 %
@Sachin Gupta
Try reading and adapting this thesis. Note, page 23 on.
http://dc.etsu.edu/cgi/viewcontent.cgi?article=3593&context=etd
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