Pcambia 1301 sequencing ?

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How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under op microscop?

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Unusual intensity drop in some sections of chromatograms in DDA?

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Can you suggest reliable sources defining "3D mesh" and "3D city models"?

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Absorption coefficient of methane?

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What is meant by baseline of FTIR data?

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Looking for help on sem image analysis?

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How to confirm the site-directed mutagenesis result without performing NGS?

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Where should the ARS sequence be introduced on a plasmid?

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Why my colony PCR results of my recombinant bacterial not showing any results?

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Interested in a SCOPUS collaboration?

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Interested in a SCOPUS collaboration?

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Transfection in HEK293T cells?

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Issues with Protein Expression in Transfected cells?

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Junjie Shao

This is the sequence of the plasmid. Generally, you just send the plasmid and primer to some seq company for seq.

https://www.snapgene.com/resources/plasmid-files/?set=plant_vectors&plasmid=pCAMBIA1301

Qurat Ul Ain Ali

Junjie Shao i am confused about primers. What kind of primers I need to design so that my plasmid can be sequenced?

which fragment do you plan to seq? the full-length plasmid?

Junjie Shao yes i want to sequence the whole plasmid. as I have to use it for further clonning