Qurat Ul Ain Ali

7 Questions 4 Answers 0 Followers

Questions related from Qurat Ul Ain Ali

Can anyone suggest Q1 journals from 7-8 IF on drought tolerance in transgenic crops?

02 August 2023 1,756 1 View

How to prepare 20% PEG 6000 in liquid MS media ?

I want to give osmotic stress to in vitro plants using PEG 6000. can anyone suggest protocol for preparing 20% PEG 6000 solution in liquid MS media? Thanks

23 March 2022 4,600 0 View

How to double digest 5 microgram DNA using Nco1 and BglII?

I have 5microgram DNA . I want to digest it with Nco1 and BglII enzyme thermoscientific through double digestion. I have not succeeded so far. Can anyone suggest optimal double digestion protocol...

06 June 2021 3,631 4 View

M13 forward and reverse sequencing primer?

I want to sequence full length plasmid pCAMBIA 1301 through M13 forward and reverse sequencing primers. I am confused about the sequence of the primers as i am doing for the first time. Can anyone...

03 May 2021 6,487 3 View

I have a plasmid of concentration 0.59 microgram/microlitre. I want 5 microgram in lyophilized form. how to get get 5microgram?

I have a plasmid of concentration 0.59 microgram/microlitre. I want 5 microgram in lyophilized form. how to get get 5microgram from 0.59 microgram per microlitre DNA conc?

29 April 2021 8,430 3 View

Pcambia 1301 sequencing ?

I got a LB plate of pcambia 1301 binary plasmid from an institute. I performed miniprep to isolate the plasmid. Now I want to sequence this plasmid. What kind of primers and reagents will be...

04 November 2020 6,213 4 View

Can I use gene specific primers to sequence my insert after TA clonning in pcr 2.1 ?

After ligating the insert in TA vector pcr 2.1, transformation and miniprep. I amplify the gene with simple gene specific primers . Now I want to sequence the insert . Which primers would I need...

01 November 2020 7,208 4 View