PAXgene blood miRNA kit with debries?

25 September 2023 0 3K Report

Hi everyone! I'm using PAXgene blood tube and PAXgene blood miRNA kit for RNA extraction from blood samples. Tubes have been stored at -80°C but when i thaw them some contain dark brown sand-like pellet/debries before starting the extraction. Does anyone have the same problem? Does anyone know what is this? I asked tech support and they said it could be fibrinogen cloth but seems unlikely to me since it is not a signle cloth rather than granular precipitate. Please let me know your experience since the extraction yield is already low and with this issue it is even worse!

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